This standard is issued under the fixed designation D ; the number mended. 1 These test methods are under the jurisdiction of ASTM Committee D06 on. ASTM D is an antifungal test method that provides a qualitative determination of fungal (mildew) resistance of paperboard, particularly those types which. Test, ASTM D Description. Standard Test Methods for Mildew (Fungus) Resistance of Paper and Paperboard. Sample retention time – 1 week.

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In many cases this will require the member to submit a significant amount of information about the product, the way it astn treated, the kind of antimicrobial used and the function the antimicrobial is expected to perform. Weekly photographic images of each sample are emailed to customers so they can follow the progress of the testing.

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Aspergillus niger ATCC is chosen from the species of Aspergillus as this species grows well on nutrient-depleted environments. This information allows the IAC laboratory to apply the correct test method and generate a test report that will provide the information requested. In this way, fungi play a major role in causing decomposition of organic material, enabling the recycling of nutrients throughout ecosystems. Mildew and Rot Resistance of Textile Materials. Fungi are grouped into four Phyla: They are microscopic fungi growing as single cells and do little damage to building products as their main source of carbon is obtained from hexose sugars such as glucose and fructose, or asgm such as sucrose and maltose.

Additional fungi are possible to be used from the cultures available in our collection.

ASTM D Antimicrobial Testing Laboratories

The Imperfect Fungi Phylum contains 25, species which do not fit into the other Phyla and are grouped together for convenience. Antimicrobial efficacy testing is performed using a variety of recognized test standards.


These enzymes degrade complex biopolymers such as starch, cellulose and lignin into simpler substances which can be absorbed by the hyphae. The fungal spores may grow on the sample only if they are capable of extracting organic carbon from the sample.

The Zygomycota Phylum contains species, including bread mold. This method covers the qualitative determination of mildew fungus resistance of paper and paperboard, particularly for antifungal treated materials. The specimens in the environmental chamber are thus surrounded with heavy fungal growth in each petri dish for 28 days to test for antifungal activity and leaching of the anti-fungal components into the PDA agar. In the ASTM D method, one test piece may be tested against three individual fungal spore suspensions or a mixture.

An uninoculated treated sample control is set up to check for presence of native fungi which may be present on the sample. Trichoderma virens ATCCformerly Gliocladium virens, is chosen from the 32 species of Trichoderma as it is adapted to thrive in diverse situations and is a soil fungus capable of inducing soft rot in wood.

ASTM D2020 – Antifungal Paper Test

Antimicrobial Textiles For Bacteria. Shubhda Research Institute offers hands-on practical training programs at different levels addressing specific industries so as to hone your skills and techniques. Air circulation within the environmental chamber circulates the fungal spores in the chamber to the vertically suspended samples four inches over the soil. The following standards have been published for testing of materials for resistance to fungi: Microbiological Testing Useful Test Reports The IAC uses standard test standards but also customizes existing test methods to meet the needs of its members so that the testing correlates to the use and abuse conditions that their treated products will be exposed to in the real world.

This fungus deteriorates plastics and paint and may present a problem in adhesive degradation.

Sixty species of Aspergillus are encountered in human pathology. Non-standard sample size depending on specific requirement of sponsor can be customized. Therefore, no organic carbon v2020 are available.


The ASTM G and TAPPI T procedures require a composite fungal spore suspension to be sprayed upon the sample materials with an atomizer while the samples are horizontal in d22020 nutrient growth agar deficient in a carbohydrate source. Locations Insights Events Careers Contact. The ASTM D standard is for fungal growth in soil, contained in the humidified environmental chamber.

In recent years, the marketplace has been inundated with fungal resistant coatings and products designed to inhibit fungal growth. Determines whether fungus will grow on a device exposed to warm, moist air in the presence of fungus spores? Aureobasidium pullulans ATCC is a staining soft rot fungus on wood products. The IAC laboratory has extensive experience in defining the appropriate test method based on the mode of action of the antimicrobial and the product being treated.

For a Quote or any clarification, Contact Us now! The IAC uses standard test standards but also customizes existing test methods to meet the needs of its members so that the testing correlates to the use and abuse conditions that their treated products will be exposed to in the real world. The test has a two week incubation period.

ASTM D – Antifungal Paper Test | Situ Biosciences LLC

Bioassays pH Simulation studies. Shubhda Research Institute Unit No. Antimicrobial Hard Surfaces For Fungi. Penicillium pinophilum ATCC axtm, formerly Penicillium funiculosum, is a fungus that deteriorates plastics.

ASTM D2020

Chaetomium globosum ATCC is commonly encountered in industrial spoilage as it grows readily on paper aatm other cellulosics. Fungi grow in the form of multicellular filaments called hyphae. Test sample size required is usually 5 x 5 cm. A connected network of these tubular branching hyphae is referred to as a colony or a mycelium.